By Alton Meister
Glutathione S-Transferases: constitution and Mechanism of an Archetypical Detoxication Enzyme (R. Armstrong).
legislation of Cholesteryl Ester Hydrolases (D. Hajjar).
the recent Biology of Adenosine Receptors (T. Palmer & G. Stiles).
Microtubule Dynamics: Bioenergetics and regulate (D. Purich & J. Angelastro).
Expression of the -Crystallin/Small Heat-Shock Protein/Molecular Chaperone Genes within the Lens and different Tissues (C. Sax & J. Piatigorsky).
Phosphoenolpyruvate Carboxykinase (GTP): The Gene and the Enzyme (R. Hanson & Y. Patel).
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And Jones. T. A.. J. Mol. Bid. 232, 192-212 (1993). 40. Reinemer, P.. Dirr. H. , L o Bello. , Federici, G . and Parker, M. W.. J. Mol. Biol. 227, 214-226 (1992). 41. , Kleywegt, G. , Mannervik. , Board, P. G . and Jones, T. A,. in Stricetitre und Function of Glictuthione Trunsferuses Tew, K . , ed.. CRC Press, Boca Raton (1993) in press. 42. , Ji. , Johnson, W. , Gilliland. G. L. , N . J. Biol. Chem. 267. 4296-4299 (1992). GLUTATHIONE S-TRANSFERASES 43 I 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54.
4296-4299 (1992). GLUTATHIONE S-TRANSFERASES 43 I 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64. 65. 43 Johnson, W. , Ji, X.. Gilliland. G . L. and Armstrong. R. N.. J. Biol. Chem. 268, 11508-11511 (1993). Armstrong, R. N.. Gilliland, G. , Ji, X.. Johnson, W. W. and Liu. f Gliitothione Tronsferuses. Tew. , CRC Press, Boca Raton (1993). , Johnson, W. , Sesay, M. , Prasad, S. M.. Ammon, H. , Armstrong, R. N. and Gilliland, C . , Biochemistry 33, 1043-1052 (1994).
Finally, NCEH activity can be inhibited by heavy metal ions at a concentration of 1 mM (39). Sodium taurocholate can protect the enzyme from inactivation by metals (44). P-chloromercuribenzoate can inhibit the activity of this enzyme at I-mM concentrations; inhibition can be reversed by reduced glutathione (39). 6 CE hydrolytic activity, Kothari et al. 4 using aorta homogenates (39). Subsequent fractionation of these homogenates using DEAE cellulose showed that the activities are separable and had different properties (43).