Advanced Time-Correlated Single Photon Counting Applications by Wolfgang Becker

By Wolfgang Becker

This booklet is an try to bridge the space among the instrumental rules of multi-dimensional time-correlated unmarried photon counting (TCSPC) and ordinary purposes of the procedure. Written through an originator of the process and by way of sucessful clients, it covers the elemental ideas of the approach, its interplay with optical imaging tools and its program to quite a lot of experimental initiatives in lifestyles sciences and scientific research.

The publication is usually recommended for all clients of time-resolved detection options in biology, bio-chemistry, spectroscopy of reside structures, dwell mobilephone microscopy, scientific imaging, spectroscopy of unmarried molecules, and different purposes that require the detection of low-level gentle signs at single-photon sensitivity and picosecond time resolution.

Show description

Read Online or Download Advanced Time-Correlated Single Photon Counting Applications PDF

Similar measurements books

Photo-Excited Charge Collection Spectroscopy: Probing the traps in field-effect transistors

Reliable country field-effect units comparable to natural and inorganic-channel thin-film transistors (TFTs) were anticipated to advertise advances in show and sensor electronics. The operational stabilities of such TFTs are therefore very important, strongly counting on the character and density of cost traps current on the channel/dielectric interface or within the thin-film channel itself.

Beam-Wave Interaction in Periodic and Quasi-Periodic Structures

The most subject of this booklet is the interplay of electrons with electromagnetic waves within the presence of periodic and quasi-periodic buildings in vacuum, in view of functions within the layout and operation of particle accelerators. the 1st a part of the publication is worried with the textbook-like presentation of the fundamental fabric, specifically reviewing straightforward electromagnetic phenomena and electron dynamics.

Observation of the Earth and its Environment: Survey of Missions and Sensors

This ebook is ready spaceborne missions and tools. moreover, surveys of airborne missions and of campaigns are available at the accompanying CD-ROM in pdf-format. in comparison with the third variation the spaceborne half grew from approximately three hundred to a thousand pages. the entire textual content - together with the electronic-only chapters - includes greater than 1900 pages.

Measuring Signal Generators: Theory & Design

The e-book brings jointly the subsequent issues:Theory of deterministic, random and discrete indications reproducible in oscillatory structures of generators;Generation of periodic indications with a unique spectrum, harmonic distortion issue and random indications with certain likelihood density functionality and spectral density;Synthesis of oscillatory method structures;Analysis of oscillatory structures with non-linear parts and oscillation amplitude stabilization systems;It considers the stipulations and standards of steady-state modes in sign turbines on lively four-pole components with unidirectional and bidirectional transmission of indications and on two-pole components; analogues of Barkhausen criteria;Optimization of oscillatory method buildings by means of harmonic distortion point, minimization of a frequency blunders and set-up time of the regular country mode;Theory of building of random sign generators;Construction of discrete and electronic sign generators;Practical layout of major devices of generators;Practical block diagrams of either analog and electronic sign turbines.

Extra resources for Advanced Time-Correlated Single Photon Counting Applications

Sample text

14 Multi-wavelength TCSPC ps Diode Laser Polychromator Single Fibre Filter 420nm LP 16-anode PMT with Routing TCSPC Module Fibre bundle Cross section of bundle Grating Sample Fig. 15 Left Optical setup for single-point autofluorescence measurement. Right Multiwavelength fluorescence decay data of human skin. Time scale 0–15 ns, wavelength scale 410– 600 nm, intensity scale logarithmic from 500 to 30,000 counts/channel 16 W. Becker Other non-scanning multi-wavelength TCSPC applications use a micro-spectrometric setup with a microscope and a confocal detection beam path to obtain fluorescence from a specific location within a cell [40].

Photon parameters available directly as digital data words are fed into the TCSPC device via the ‘Channel’ register. Examples are the wavelength channel in which a photon was detected by a multi-wavelength detector (see Sect. 1), or the number of one of several multiplexed lasers or laser wavelengths (see Sect. 2). The external data word is read into an the ‘Channel’ register in the moment when the corresponding photon is detected. Additional parts of the address can be generated by a ‘Sequencer’ block.

2 would correspond to a detection rate of 16 MHz. This is much more than the detection rates in typical TCSPC experiments. With state-of-the-art laser sources and TCSPC electronics the count rate—and thus the available acquisition speed—is rather limited by the photostability of the samples than by pile-up effects [22]. This is especially the case in fluorescence lifetime microscopy where the fluorescence comes from a pico- or femtoliter sample volume. The real limitation of the classic TCSPC principle is that it is intrinsically onedimensional.

Download PDF sample

Rated 4.12 of 5 – based on 26 votes